Optical microscopy 1100-OM
1. Microscopy contrasts, applications, and challenges; layout of a microscope.
2. Basic concepts, selected types of optical microscopes.
3. Monochromatic field propagation, diffraction.
4. Field propagation through a lens, imaging.
5. Interference-based imaging techniques: optical coherence tomography, holographic microscopy.
6. Fluorescence.
7. Intensity and coherence propagation, light detection.
8. Three-dimensional imaging.
9. Confocal microscopy.
10. Two-photon microscopy.
11. Coherent nonlinear microscopy.
12. Structured illumination microscopy.
13. Super-resolution microscopy: stimulated emission depletion.
14. Super-resolution microscopy: localization.
15. Summary: How to build a microscope.
Main fields of studies for MISMaP
Course coordinators
Mode
Blended learning
Learning outcomes
The student will understand the physical foundations and the main techniques of optical microscopy. She/he will be able to identify the limitations and the essential technical aspects of the main optical microscopy techniques.
Assessment criteria
An oral exam covering the material discussed during the lectures.